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Missing only tAg failed to replicate to detectable levels in PHFG cells. By modifying the replication assay and increasing expression of the JCV early proteins under the control of more potent CMV transcriptional signals, low levels of JCV DNA replication (,10 of wild type; Fig. 8) were observed; a construct lacking tAg and the three T9 proteins, however, still failed to replicate under these con

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