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L standard). Samples had been evaporated to dryness (EZ2-Bio, GeneVac), as well as the remaining residue was saponified at 90 for 60 min in two mL of 6 (w/v) KOH in MeOH. Upon cooling to area temperature, 1 mL of hexane and 1 mL of H2O have been added, and also the mixture was shaken vigorously for 20 s. Following centrifugation (three,000g for two min) to separate the phases, the hexane phase w

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