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Depilatory cream. Twenty-four hours later, 5 105 PFU of HSV-1 was applied to the depilated flank approximately 3 mm ventral to the spinal column, and the skin was scratched with a 27-gauge needle, using 20 horizontal strokes and 20 vertical strokes over an approximate area of 3 by 3 mm. The flank was observed daily for at least 10 days, and cumulative scores for primary and secondary areas were re

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